NSFC-31471512

国家自然科学基金面上项目

玉米幼胚胚性愈伤组织诱导能力全基因组遗传解析，国家自然科学基金面上项目，2015.01-2018.12，主持；

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执行时间：2015-01 至 2018-12

中文关键词 玉米幼胚；胚性愈伤组织；诱导能力；遗传解析；全基因组

英文关键词 Maize embryo; Embryonic callus; Induction ability; Genetic analysis; genome-wide

中文摘要：玉米胚性愈伤组织诱导是转基因技术的关键环节，我国高胚性愈伤诱导率骨干自交系匮乏，严重阻碍了转基因育种的快速发展。本项目拟采用高覆盖率重测序构建的玉米IBM连锁图谱及全基因关联分析，深入挖掘与玉米高胚性愈伤诱导率显著相关的SNP标记和QTL位点，验证候选基因功能。首先以包含280份DH系的IBM Syn10群体及其构建的6618个bin marker基因型数据为基础，结合多年多点表型鉴定精细定位高胚性愈伤诱导率QTL区间，发掘控制玉米高胚性愈伤诱导率的关键候选基因。再利用先锋公司开发的高密度SNP芯片对300份玉米自交系进行全基因组关联分析，鉴定与高胚性愈伤诱导率显著相关的SNP位点和关键基因。并利用比较基因组、生物信息学手段，确定并克隆优良等位基因，进而通过转基因和TALEN定点突变验证候选基因功能。结合前期对胚性愈伤诱导过程基因表达调控研究，对玉米胚性愈伤诱导能力进行遗传和调控网络解析。

英文摘要：The induce process of embryonic callus from maize embryo is the core technology in GMO industry. But due to the lack of core inbred lines with high induction efficiency for embryonic callus, the development of maize transgenic breeding was inhibited seriously. In this study, we will combine the high genetic resolution map in IBM population and genome-wide association mapping study, identify and verify the candidate genes with highly significant SNPs and QTLs which related to the trait of high induction efficiency of maize embryonic callus. First of all, we will generate a high density genetics map with 6618 bin markers in a 280 lines IBM Syn10 DH population, with mixed different environment and locations phenotype data to fine mapping the major QTLs. Meanwhile, we are going to use high-resolution SNP microarray for further association study in a 300 hundreds inbred lines population, for the goal of identifying significant SNPs and important candidate genes with the collaboration with DuPont Pioneer. By the method of comparative genomics and bioinformatics, we aim to identify excellent alleles. We also plan to clone these genes from the maize inbred lines with high induction rate of embryonic callus.Then, the functions of the above cloned genes will be validated by both transgenic and TALEN technologies. Combined with previous researches on microRNA regulation and gene expression of embryonic callus induction process, the project will be intended to construct genetic and regulatory networks of induction ability for maize embryonic callus on the whole-genome level.

成果产出：

  1. 专利：玉米组织培养苗床 作者：沈亚欧;刘敏;胡红梅;龙芸;王艳丽 专利编号：201820093719.8 授权机构：中华人民共和国国家知识产权局 授权日期：2018 授权区域：中国
  2. 专利：玉米幼苗水培箱 作者：沈亚欧;卿春燕;梁天虎;郑艳;马浪浪 成果类型：专利 专利编号：201820092318.0 授权机构：中国国家知识产权局 授权日期：2018 授权区域：中国
  3. 玉米胚性愈伤组织诱导相关基因GRMZM2G020814的SNP分子标记及其应用 作者：沈亚欧;龙芸;潘光堂;王磊;张晓玲;马浪浪;杨云 成果类型：专利 专利编号：201710287129.9 授权机构：中华人民共和国国家知识产权局 授权日期：2017 授权区域：中国
  4. Haploid strategies for functional validation of plant genes 作者：Yaou Shen;Guangtang Pan;Thomas Lubberstedt 成果类型：期刊论文 收录情况：SCIE 期刊名称：Trends in Biotechnology 卷：33#10 发表日期：2015 浏览次数：1 次 页码：611#620
  5. Genome-wide analysis of transcription factors involved in maize embryonic callus formation 作者：Ge Fei;Luo Xu;Huang Xing;Zhang Yanling;He Xiujing;Liu Min;Lin Haijian;Peng Huanwei;Li Lujiang;Zhang Zhiming;Pan Guangtang;Shen Yaou 成果类型：期刊论文 doi：10.1111/ppl.12470 收录情况：SCIE 期刊名称：Physiologia Plantarum 卷：158#4 发表日期：2016 浏览次数：1 次 页码：452#462
  6. Transcription Factors Responding to Pb Stress in Maize 作者：Zhang Yanling;Ge Fei;Hou Fengxia;Sun Wenting;Zheng Qi;Zhang Xiaoxiang;Ma Langlang;Fu Jun;He Xiujing;Peng Huanwei;Pan Guangtang;Shen Yaou 成果类型：期刊论文 doi：10.3390/genes8090231 收录情况：SCIE 期刊名称：GENES 卷：8#9 发表日期：2017 浏览次数：2 次 页码：0#231